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KMID : 0616120070380020192
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Korean Journal of Pharmacognosy
2007 Volume.38 No. 2 p.192 ~ p.196
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(HPLC Analysis of Saponins in Platycodi Radix)
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Kim Hyun-Gi
Choi Jae-Seok
Yoo Dae-Seok
Choi Yeon-Hee
Yon Gyu-Hwan
Hong Kyung-Sik
Lee Byung-Hoe
Kim Hae-Jin
Kim Eun-Ju
Park Byoung-Keun
Chung Young-Chul
Kim Young-Sup
Ryu Shi-Yong
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Abstract
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A rapid and practical HPLC assay was developed for quantitative analysis of saponins in Platycodi Radix. Seven saponin components in Platycodi Radix, i.e., deapioplatycoside E, platycoside E, deapioplatycodin D_3, platycodin D_3, polygalacin D_2, platycodin D_2 and platycodin D were successfully resolved on C18 column and detected by ELSD. Standard curves were linear over the concentration range 1 {sim}2,000 {mu}g/ml (r2>0.992). Intra- and inter-day coefficients for variation of seven saponin components were < 10% and limit of quantification of them were around 0.7{sim}1.5 {mu}g/ml, respectively. Using this method, contents of seven saponins in various plant materials under different cultivating conditions were estimated.
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KEYWORD
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Platycodon grandiflorum, campanulaceae, saponin, HPLC, quantitative analysis
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